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Which Detergent Extracts Strawberry DNA The Best? (page 2)

based on 15 ratings
Author: Mary T.

Experimental Procedure

To perform my experiment I completed the following steps:

  1. Add 4 oz of distilled water to the beaker
  2. Add 1 ¼ oz (6.25 ml) of powdered buffer into the beaker
  3. Stir until buffer is dissolved in distilled water
  4. Add 5 ml of detergent to mixture, and stir for 2- 4 minutes, slowly (Try not to create suds)
  5. Put mixture into ice and chill
  6. Mash 5 strawberries in a blender
  7. Warm up a cup of hot water at 60 degrees Celsius
  8. Put “mush bag” into water
  9. Set it in hot water for 10 minutes
  10. Put 5 tbsp (75 ml) of mush into a beaker
  11. Put 10 tbsp of chilled buffer into beaker
  12. Stir mush with chilled buffer for 4 minutes
  13. Pour the mush into the beaker with the filter
  14. Add 5 ml of “liquid from mush” into each of ten test tubes
  15. Add 5 more ml of alcohol to test tubes
  16. Put 1 drop of Blue DNA Stain into each beaker
  17. Observe white, slimy DNA
  18. Measure your results

During my experiment the following made up my controls:

  1. Strawberries
  2. Distilled water
  3. Powdered Buffer

The following was my variable:

  1. Type of detergent

I performed the following three sets of experiments 10 times:

  1. DNA extraction with Ajax dishwashing liquid
  2. DNA extraction with Purex laundry detergent
  3. DNA extraction with Fructis shampoo

Results

The ten Ajax experiment trials resulted in measured extractable DNA from 0.250 to 1.500 milliliters with an average extracted DNA of 0.775 milliliters. The use of Ajax produced the most consistent results. Six of the ten trials produced 0.750 milliliters of visible DNA. 

The ten Purex experiment trials resulted in measured extractable DNA from 0.250 to 1.750 milliliters with an average extracted DNA of 0.625 milliliters. Out of all 30 trials for all three detergents, one of the Purex trials had the highest amount of visible DNA, which was 1.750 milliliters. Even though Purex produced one of the largest quantities of visible DNA in one trial, the remaining nine trials did not produce consistent results. 

The ten Fructis experiment trials resulted in measured extractable DNA from 0.100 ml to 1.500 milliliters with an average extracted DNA of 0.485 milliliters. Two of the Fructis trials had the lowest amount of visible DNA, which was 0.100 milliliters. The Fructis trials produced the same inconsistencies similar to the Purex trials.

 
 
 
 
 
 
 

Trial #

Ajax

Purex

Fructis

 
 
1
0.750
0.500
0.250
 
 
2
0.500
1.750
0.100
 
 
3
0.750
0.250
0.150
 
 
4
0.750
0.250
0.100
 
 
5
0.750
0.500
0.500
 
 
6
0.750
0.250
0.250
 
 
7
1.000
0.250
1.250
 
 
8
0.750
0.500
0.250
 
 
9
0.250
1.000
0.500
 
 
10
1.500
1.000
1.500
 
 
 
 
 
 
 
 
Avg DNA ml
0.775
0.625
0.485
 
 
 
 
 
 
 

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