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The Effect of Glucose and Sucrose as Dietary Additives on the Lifespan of Wild-type and GAPDH Mutant Caenorhabditis Elegans (page 3)

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Author: Megan S.

Questions for Future Research 

The next step will be quantifying the possible GAPDH inhibition resulting from added sugar in the C. elegans’ diet. This could be done using either real-time polymerase chain reaction (PCR) to use gene expression to quantify the GAPDH inhibition or using zymography, which would utilize GAPDH’s substrate to quantify this possible inhibition. While this experiment indicated that glucose and sucrose cause a decrease in C. elegans’ lifespan, the data were solely quantitative and focused on the length of life. Because other factors besides length of life, such as quality of life and strength of bodily functions, are important when studying longevity, future

 experiments should investigate both the quality and length of life. Future experiments could also investigate the effect of other sugar-like substances such as fructose, corn syrup, and artificial sweeteners on C. elegans’ lifespan and GAPDH inhibition. This experiment utilized wild-type C. elegans as well as GAPDH mutant C. elegans; future experiments could investigate the effect of sugar on the lifespan of C. elegans with other mutations. These results could help scientists better understand the effect of certain genes on aging and the metabolism of sugar-like substances. The effectiveness of antioxidants in preventing aging has been challenged in the controversy over the role of oxidative stress in aging. Thus, future experiments could use C. elegans grown in cultures with antioxidant additives to investigate antioxidants effect on aging.

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