The Lipase-Bile Relationship in the Digestion of Fats & Oils (Lipids) (page 2)

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Experimental Procedure

  1. Fill three jars or plastic bags with water at room temperature. Add a few drops of oil to each.
  2. The first jar will serve as the control because it will only contain oil and water.
  3. Add ¼ teaspoon (1 ml) of bile salt to the 2nd jar or bag.
  4. Add ¼ teaspoon (1 ml) of bile salt and ¼ teaspoon (1 ml) powder lipase to the 3rd jar or bag.
  5. Label the jars based on what is in them.
  6. Sir or shake (if using a bag) the content of all three jars or bags and record what happens.
  7. Wait 20 minutes, then observe the mixtures again and record what was seen.
  8. Use terms such as “layers,” “little droplets,” “very tiny drops,” “uniform mixture,” etc when recording the observations.
Test Samples    


    Initial Time
      20 minutes Later

Water + Oil (Control)


Bile + Oil + Water


Bile + Oil + Lipase + Water

Go to the Internet download and print an image similar to the one shown (or use this image). Using colored pencils trace the journey of bile from the gall bladder along with lipase from the pancreas to where they combine to break-down lipids for absorption into the body.


The Digestive and Excretory Systems, Susan Dudley Gold, Enslow Pubications Inc., ISBN:0766020223, 9780766020221, 978-0766020221 

This book gives a breakdown and description of all of the body parts, organs, and fluids that are involved in the digestive system and how everything ties together. The book consists of text supported by color illustrations and graphics that facilitate conceptual understanding of content. Other features found on the book include chapter notes, a further reading list, Internet addresses, a glossary, and an index. 

ABC of Liver, Pancreas and Gall Bladder, I J Beckingham, Bmj Publishing Group, ISBN: 0727915312 

This book is covers the medical and surgical problems affecting the liver, pancreas and biliary system. The material in the book is comprehensive, written for medical and nursing students, GPs and junior hospital doctors in general medical and surgical training however; the young investigator and his or her parents (teachers) can use this book as a general reference resource. Note: This book is very expensive to purchase and therefore should be consulted at the local public library or medical university library.  

The Digestive System, Carol Ballard, Heinemann Publications, ISBN: 1432934309 

This book explores the structure of the digestive system and how the body digests and uses food. It explains the different illnesses and conditions that can affect human digestion, from indigestion to irritable bowel syndrome, and outlines ways that the digestive systems can be kept healthy-including the importance of a balanced diet.

Fat and Bile Digestion Animation:


What is Lipase? 

Note: The Internet is dynamic; websites cited are subject to change without warning or notice! 


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