Genetics of Bacteria Review Practice Problems
Review the following concepts if needed:
- Genetics of Bacteria for Genetics
- Bacterial DNA Replication and Cell Division for Genetics
- Bacterial Transcription for Genetics
- Bacterial Translation for Genetics
- Genetic Recombination for Genetics
- Regulation of Bacterial Gene Activity for Genetics
- Mapping the Bacterial Chromosome for Genetics
Vocabulary For each of the following definitions, give the appropriate term and spell it correctly.
- Descriptive of all mutant strains of bacteria requiring supplementation to minimal medium for their growth.
- A colony of genetically identical bacterial cells.
- The Greek letter representing an intermediate structure of the bacterial genophore when it is halfway through bidirectional replication from a single origin.
- A solid mass of bacterial cells covering the surface of nutrient agar medium in a Petri dish.
- A form of bacterial recombination requiring cell-to-cell contact.
- A form of genetic recombination between bacterial cells that is mediated by a bacteriophage.
- A partially diploid exconjugant.
- A small, circular DNA molecule capable of replication independent of the genophore in its bacterial host.
- The bacterial topoisomerase that pumps negative supercoiling into relaxed DNA circles.
- The protein that unwinds double-stranded DNA at each replication fork.
Multiple-Choice Questions Choose the one best answer.
- Which of the following is classified as a prokaryote? (a) protozoa (b) yeast (c) bacteria (d) algae (e) more than one of the above
- Which of the following is found in prokaryotes? (a) mitochondria (b) histones (c) actin and myosin (d) formylated methionine (e) more than one of the above
- The physiologically receptive state in which a bacterial cell is able to be transformed is called (a) sensitized (b) activated (c) competence (d) lysogenic (e) inducible.
- Which of the following is not a method for gene transfer in bacteria? (a) translocation (b) conjugation with Hfr transfer (c) transformation (d) transduction (e) conjugation with an F' cell.
- Which of the following modes of cell division or DNA replication is not used by bacteria? (a) binary fission (b) rolling circle (c) theta replication (d) mitosis (e) bidirectional replication
- The distances between bacterial genes, as determined from interrupted conjugation experiments, are measured in units of (a) recombination (b) nucleotide pairs (c) minutes (d) micrometers (e) percentage of genome
- The subunit of RNA polymerase involved in initiation of bacterial transcription is designated (a) alpha (α) (b) beta (β) (c) gamma (γ) (d) sigma (σ) (e) rho (ρ)
- A Shine-Delgarno sequence (a) serves as a binding site for bacterial ribosomes (b) is involved in bacterial transcription (c) forms part of mRNA trailers (d) serves as a recognition site for stopping RNA synthesis (e) serves as a recognition site for termination of translation
- The type of gene regulation governing the lactose operon of E. coli that involves the lac I gene product is best described as (a) positive, inducible (b) negative, repressible (c) attenuation (d) feedback inhibition (e) negative, inducible
- The ineffectiveness of many antibiotics today is most closely associated with (a) bacteriophages (b) F plasmids (c) R plasmids (d) catabolite repression (e) bacterial transformations
- theta (θ)
- DNA gyrase
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