Does 100% Fruit Juice Have More Vitamin C than Juice with Artificial Ingredients?

based on 52 ratings
Author: Sofia PC

Grade Level: 8th - 10th;  Type: Health Science/ Food Science/Chemistry


We will find out whether 100-percent fruit juice has more Vitamin C than juice with artificial ingredients.

Research Questions:

When one thinks of Vitamin C, pictures of oranges usually come to mind. That's because vitamin C is highly abundant in citrus fruits such as oranges, lemons, limes, and grapefruits. Does juice made from these fruits naturally have more Vitamin C than artificial fruit juices? In this experiment, we will be using three different kinds of orange juice: from concentrate, not from concentrate, and an orange-flavored drink with artificial sweeteners/flavors (Sunny D).


  • Orange Juice
  • From Concentrate (like Minute Maid brand) Not From Concentrate (like Tropicana or Florida's Natural brand)
  • Orange-flavored Drink (like Sunny D)
  • Cornstarch
  • Iodine
  • Water
  • Beaker (if you are going to use a burner); Pot (if you are going to use a stove)
  • Eyedropper/pipette
  • Several test tubes w/ rack
  • A white piece of paper
  • Pen and paper for notes

Experimental Procedure:

Preparing the Iodine Indicator

  1. Mix cornstarch (one tbsp) and water together to make a paste.
  2. Add 250ml of water and boil for about five minutes.
  3. Using a dropper or a pipette, add 10 drops of the boiled solution to 75ml of water.
  4. Add iodine to the mixture until it turns a dark-purple color.

Comparing Vitamin C Levels

  1. With a dropper, add 5ml of the iodine indicator solution to a standard 15ml test tube. Using a clean dropper (to prevent contamination), add 10 drops of the orange juice from concentrate into the test tube.
  2. Clean the dropper and do the above for the “not-from-concentrate” juice sample as well as the orange-flavored drink.
  3. Compare which one turns a darker color. The darker color means there is less Vitamin C present in that particular sample.

Terms/Concepts: Vitamin C; Metabolic function; Heat; Cooking; Necessary functions of organisms


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